Well, your cell expansion is very clearly not small enough to just include the nucleus. It should be the same as your pixel size from the Image tab. I still see a significant expansion, which you indicated you didn’t want.
Your threshold should also not be 0.1, as that is way too low. For Subcellular detections, you can select a single cell and keep hitting “Run” to find out what threshold you should be using for your image. Keep increasing it until you get what you are expecting. I figure it will be higher than 0.3. Maybe as high as 0.7, depending on your background.
Neither of those is an original image, though, so I can’t really help more than that. I can’t work with a snapshot.