Counting stained nuclei

Hello!
I am very new to this program and would be grateful for the help. I need to count the number of nuclei that are staining brown. Please see attached image. How do I do this

Hi,
I’ve posted a pipeline that should get you started. You can take a look at the module notes at the top of the settings panel to get my thought process. Hope this helps!
-Mark
2012_11_07.cp (6.68 KB)