Counting Spots in ELISPOT Images

Is there a way to automate the counting of spots in a given ELISPOT image? I have tried using ‘Analyze Particles…’ and the plug-in SpotCaliper, but they provided inaccurate results.

Thanks for your help.

Pep%201%20CD8%20%20Leukemia

Maybe try something like this: Finding tire rubber in low quality images
Though it really looks like that should be doable with a fairly simply FIJI filter, despite being very blurry. If the image can be taken better, and at higher resolution, your results should be more accurate. I think there is a watershed function within Weka that can be used to split the spots, though how effective it is will depend on picture quality.

1 Like