My lab typically uses brightfield images (admittedly not what CP was optimized for) but I’ve had success particularly through using the UnmixColors module. I’m currently having some issues with analyzing images in which the targets to be quantified are small and fairly rare (often under 10 per image). When I try to select these objects, CP always overselects as if it’s expecting more objects. Any recommendations?
Images linked; features of interest are the bright pink/magenta punctate signals against the hematoxylin counterstain.