I am struggeling with the following problem: I simply want to identify cells by DAPI (Blue channel) + Phalloidin (red Channel) Staining and count them. Additionally I’d like to count those of the identified cells labelled with a green fluorescence staining (green channel).
Here is my pipeline so far Pipeline 03122020.cpproj (706.0 KB).
Can anyone help me to improve my pipeline to get a percentage of positive green cells in ratio to all cells?
Thanks a lot