I’m new to image J and i’ll try to explain my problem as precisely as i can.
I have to analyse cells on a rat brain image and they appear as black dots on the brain.So i turn my picture into a 8 bit one and use the threshold to make them red.
But when i use the threshold to make them red, some stains on the image and on the brain also become red.
Is there a way to threshold only certains pixels wide things on image J? Or to isolate the little black dots?
Because as far i as know about image J i have to use the paintbrush tool to clear all the stains pixel after pixel.