I’m trying to count the # of red foci per blue nucleus for this image here.
And the problem is that on imageJ it overestimates the area of the nucleus because it thinks the surrounding red dots around the nucleus is also part of the nucleus, as imageJ converts images to black and white
I did the following to try to do automated counting of nucleus:
-Plugins > Feature Extraction > Feature J Lapalcain
-Image > Adjust> Threshold
-Process > Binary > Make Binary
-Process > Binary > Fill Holes
-Process > Binary > Dilate
-Watershed (what I’m going to do next…)
As you can see in this picture, the nuclei nuclei appear to be larger than they actual should be (compare with the blue nuclei in the colored picture), because imageJ erroneously involve the red dots surrounding the nuclei also. How can I prevent this from happening? I only want to count the blue area and disregard the red area. I would really appreciate your help. Thank you.