I run a timelapse confocal experiment of three days in which over time my cells in culture recombine acquiring nuclear red fluorescence.
I was wondering if there is any way to quantify (to count) automatically the number of red cells that appear over time. Of course the idea is cells are counted the moment they appear and then not count again. I understand it is a tricky quantification because cells move and they divide spreading the fluorescence to the new progeny of cells. But maybe someone knows how to do it?