I am trying to asses the number of goblet cells in respiratory epithelium using H&E slides. Alternatively the slides are stained with PAS or Alcian blue which stains the cytoplasm of the goblet cells red or blue.
Would anybody have any experience in doing this and would have a pipline to do this or would be willing to think along?.
!!!Apologies, just realised that I need to upload a usable image, will do so tomorrow!!!
To give you an idea of what I’m looking at, the images below are non usable examples
PAS stained goblet cells (pink/red):
Alcian Blue stained goblet cells