Hello, I’m just wondering if someone could give some advice on using Cell Profiler to count the number of fluorescent foci present per bacterial cell in an image.
I need to be able to identify cells, and count how many GFP-tagged protein ‘spots’ are present in each cell, and having looked at the Speckle Counting example on this website, it seems possible. Up until now, I have been doing this both manually (very time consuming for hundreds of images!) and by writing scripts in imageJ but so far this has proven difficult and unreliable. If Cell Profiler can do this for me, could someone give me some pointers on how best to adapt the Speckle Count script to suit the images and cells I am working with?
I have attached a bright field and its respective GFP example images showing what I need to count, which I hope will make things clear (they may need to be dowloaded and opened in imageJ as for some reason they’re appearing as black when uploaded)
Thanks to anyone who can provide assistance!
Centre for Bacterial Cell Biology
Institute of Cell and Molecular Biosciences