I am currently trying to count the number of dividing cells,that are fluorescent (green), in ovary tissue. To obtain a percentage of dividing cells I use DAPI as a stainer for all the cells nuclei. My pipeline is working fine with the DAPI counting but not great with the dividing ones, specially when there are not a lot of dividing/fluorescent cells. Maybe because the automatic detection based on intensity is not the good one, but I can’t get any other to work better.
Attached is my pipeline and a set of the pictures I am using.
Thanks a lot for your help !
PS: I use crop to be able to isolate only one follicule before counting the cells.
PipelineF.cpproj (532 KB)