I’m an undergraduate student and I am trying to use ImageJ to count the number of fluorescent bacteria cells flowing into and out of a device in a microfluidic channel.
Attached below is a movie file of the inlet of the device that I have converted into an 8-bit TIF format. The device is out of frame just on the right side of this image sequence.
My goal is to be able to count the number of fluorescent bacteria cells that are flowing into and out of the device (movie above only shows the inlet flow).
Right now, I’m relying on the plugin called ‘Mosaic’ that allows for particle trajectory tracking and counting. Basically, I am currently relying on counting the number of trajectories. However, I find that this method gives inaccurate results as there are problems with trajectory linking.
What is the best way about doing this?
Thanks in advance!