I want to count mosquito eggs using ImageJ instead of by hand, however I can’t seem to get the settings right. I already googled and checked some other topics, sorry if one has not caught my attention yet!
Some background information:
I use Windows 10. I am taking a picture with a simple compact camera, mostly 1x or max 2x zoom in the same tube. Mosquito eggs from the Anopheles genus are dark, small ellipses that are scattered over the water surface. I am taking a picture on a white surface but some dark stains from the mosquito are also in the water.
This is how I get my picture out of the camera:
I cropped it and tried to remove the stains by painting them white by hand (but preferably I would not do that since I have many pictures).
The main problem with my masks is that these eggs can lay pretty closely together and therefore do not seem to be recognized anymore. Below I have an example, where only 38 out of the 74 eggs are counted. You can see that the clumps of eggs are missed.
Thresholds seem to be sufficient but it cannot translate all separate eggs. For instance, the Y-shape at the bottom right is 4 eggs in total.
Attached another picture of a raw image just in case (the one from the Y shape).
I don’t know how to copy a whole timeline in here, sorry, but I hope that my current information is sufficient…
Please let me know if there are any questions or if anything’s unclear! I’m looking forward to your feedback on how to use this.