Thank you so much for your help and quick response!
I tried IdentifyObjectsManually but I realized that, as you said, I would have to trace each nuclei individually. Is there a modified version of it where I can trace the desired epidermal region, and then I can apply “IdentifyPrimaryObjects” to this specified region? Or is there a way to crop the image so that it only includes the epidermis?
I have included a sample picture of DAPI staining where the epidermal region which I want to quantify is traced in red. There is greater intensity here, since the cells are more squished together. Is there a way to use that maybe to detect if it is the epidermis or not? (Like checking if the distance between cells exceeds a certain threshold?)
Thank you again for your help! I am really new to this and I appreciate the support.
(I cannot use pictures from my own lab so I used an image from the paper " TRPV3 Channel in Keratinocytes in Scars with Post-Burn Pruritus", I hope that’s okay!)