I’m very pleased to start using the CP. But as expected for a beginner i have had lots of challenges to put my pipeline to work.
I give some small bugs to cells to evaluate the phagocytosis.
The objective: simply know an infection index (cells with intracellular bugs)
The method: the bugs are GFP, the non-phagocytosed bugs are imunostained, the cells can be identified in the phase image. So i have 3 images per set.
I could identify all three objects independently, the problem is how to count per image the cells hosting phagocytosed bugs. To do that I need to count the cells that colocalize the only GFP bugs (not immunostained). The object for immunostaining are a bit bigger than GFP signal.
Please, could you help me.