I have been trying to count and quantify two distinct cell populations (CD68, CD90) from IF photographs. The idea is to quantify the total number of CD90+ and CD68+ cells and in addition determine the number of CD90+ cells that are directly associated with CD68+ cells. Additionally, I would laso like to modify the pipeline to include a measurement (distance in pixels) from a CD90+ cell to the nearest CD68+ cell.
I have been using the follwing modules in my pipeline:
LoadImages (to load the CD90 file),
IdentifyPrimaryObjects (to identify the CD90 objects),
IdentifySecondaryObjects (to identify the CD68 objects in a 10px radius),
ExportToSpreadsheet (to collect data).
I know the distance parameter is semi-ideal and should better be set as propagation. However, my main issue is with the count itself. All of my files give me the same counts for both populations, which is not possible. Also, I haven’t found a way to address the distance in pixel problem.
I would apppreciate any suggestions of pipelines that I could try. Unfortunatly, I know too little about CP to take advantage of its capabilities beyond basic counting.
Please find my test files below.
Thanks in advance.
13-04-15 test pipeline.cp (6.98 KB)