I have a small issue, I hope you can help me with. See the ImageJ-processed picture below. It has several stained (DAPI) cells in a 8-bit image. The staining efficiency is not that good because of some practical reasons.
Anyway, I want to count them. By eye it is clearly see-able, which parts are cells and which not. The problem is that with the threshold I cannot highlight the right parts. This is because in some parts the “background” or the space between two cells is brighter than in other parts the cells themselves. I wanted to use Threshold + watershed + Analyze Particles.
Do you have an idea how to deal with that?
Thanks in advance.
PS: This image is already processed: background is subtracted, brightness etc. is improved. Too high and too low values are excluded (with over/under and Huang). The high values came from dead cells wandering around.
20200331_100%-1_corrBackground-2.tif (5.6 MB)