I have done a Transferrin Uptake Assay in BT549 breast cancer cells using Transferrin-Alexa488 and I have quantified them using ImageJ and mean fluorescent intensity per cell.
I want to now try and quantify the amount of particles per cell but I am having a difficult time. I cannot find any plugins or macros for ImageJ but have been told to try CellProfiler.
I tried CellProfiler and
I have attached one of my images (it is a dual layered .tif file). I have attempted to use this image with the “Cell/particle counting, and scoring the percentage of stained object” pipeline,
as seen on this webpage
But it does not seem to work on my images.
I was wondering if you could provide any guidance on the best approach to quantify the number of particles in each cell of my images.