I am a beginner in using QuPath.
I have IHC images stained with hematoxylin nucleus stain combined with a double stain ( a purple for CD45 stain and a yellow for aSMA staning). Under section: Brightness and color I can add colors for hematoxylin, and eg yellow, but i need and additional color option so i also can choose only to see purple (CD45 positive cells). How can this be done ?
Additionaly, the cells iam looking at are CD45 positve in the nucleus and SMA postive in the cytoplasm. Any ideas of how to develop a method for detection of theses cells, that have both purple and yellow.
Thanks in advance for any help.