I’m trying to create a pipeline to count and calculate the percentage of positive stained cells (intracellular) in ratio to all stained nuclei (DAPI) in a immunofluorescence staining experiment.
I already figured out to identify respective objects. now I’m struggeling with the modules and settings to count those objects and the calculation.
I used IDENTIFY_PRIMARY_OBJECTS, which does a good job.
Can anyone please help me? Which modules to I have to add with which settings?
Thanks a lot