Hello,

I wonder if anyone can help me… I have been looking for correlations between certain biological properties of colonies of a primary human cell line and the size, shape, cell density etc. of these colonies - probing the latter using cell profiler. There is an inverse correlation with my property of interest and the ‘eccentricity’ of the colony shape suggesting circularity is relevant, but there is also a correlation with certain zernike features. Specifically, zernike ‘6 6’ for which there is a good negative correlation over the range 0.002 - 0.011. There is also a negative correlation with the sums of the zernike features (if that means anything) over the range 0.460-0.581. I’ve copied and pasted the list of zernikes with the corresponding R2 value for the correlation with my property of interest at the bottom of this post. I’ve also attacher an image of the 12 colony shapes ranked according to my property of interest .

I know multiple comparisons such as I’ve done are statistically dodgy but it would help me weigh up whether the correlation is interesting or not if I knew what it meant…! I’ve been browsing lots of articles but I have to say that they are all a bit beyond me. Any pointers would be much appreciated (especially if they come without equations!)

thanks

jon

and thanks to the cellprofiler team for an awesome resource!

1 1 …0.017536974

2 0 …0.118418771

2 2 …0.238829273

Sum of 2 …0.340868173

Sum of 1&2 …0.337366893

3 1 …0.275595982

3 3 …0.002391311

Sum of 3 …0.2083681

Sum of 1-3 …0.458606822

4 0 …0.463725234

4 2 …0.279502328

4 4 …0.265158976

Sum of 4 …0.080721624

Sum of 1-4 …0.302732752

5 1 …0.006461294

5 3 …0.03418431

5 5 …0.025478357

Sum of 5 …0.006509283

Sum of 1-5 …0.321657119

6 0 …0.079320561

6 2 …0.003114777

6 4 …0.227566627

**6 6 …0.593296892**

Sum of 6 …0.341169631

**Sum of 1-6 …0.627433229**

7 1 …0.001013277

7 3 …0.000131616

7 5 …0.034213096

7 7 …0.146625932

Sum of 7 …0.000474104

**Sum of 1-7 …0.510500777**

8 0 …0.244045533

8 2 …0.002000277

8 4 …0.196964253

8 6 …0.119374538

8 8 …0.042992997

Sum of 8 …0.004463649

Sum of 1-8 …0.436617994

9 1 …0.080625903

9 3 …0.079641718

9 5 …0.072705498

9 7 …0.011855241

9 9 …0.255323243

Sum of 9 …0.431769649

**Sum of 1-9 …0.520728336**