I’m currently using CellProfiler to develop a cell measurement protocol. My questions are; (1) Is the conversion of 16 or 24 bit images to 8 bit the standard for CellProfiler (or other imaging software) to make measurements such as identify primary objects (nuclei)? (2) Can CellProfiler make measurements in 24 bit format? (3) Is there significant image information loss when 24 bit images are converted to 8 bit? If, so how does this affect CellProfiler’s ability to identify objects?
Thank you for your help.