Updated from original
I have begun to understand signed and unsigned image data. The data is collected in ScanImage as 16-bit signed to include noise in the histogram, as noise can be centred around low negative values/zero. I think the previous versions of this software that I have used collected data as unsigned 16-bit.
I am running in to a problem with plugins such as MultiStackReg, which pad the image with zeros, but in this case, the true zero is -2^15 (half of 16-bit). So once the image has been registered I have a problem with my LUTs trying to auto-contrast to include these negative values.
Is there an easy way to convert the data from signed 16-bit to unsigned 16-bit?
I’ve converted to 32-bit and back to 16-bit and this seems to solve the problem, I’m not entirely sure, but is that a viable solution?