Does anyone know if there is a way of compressing a z-stack, but keeping it intact as a stack please? I have a lot of very large images (some as big as ~40 Gb) which consist of a couple of hundred confocal tiles stitched together, and 10-20 z planes per tile. Such large files are unwieldy, and I cannot cut them up because I’m tracing neurons (in a different programme - Neurolucida) that span the entire image.
I can save each individual z plane as a JPEG or PNG using Save as → Image Sequence, and then use Images to Stack to reconstruct a stack, but it seems inefficient. Is there a better way?