First, thank you all for building a wonderful program.
I’ve been easily able to understand how to obtain the “mean intensity” of my fluorescence images however I’m having difficulty navigating through another task.
I have two pictures of identical sections- in one histones are stained with red fluorescence while the other has protein stained with green fluorescence. I’m looking to find an inverse correlation between the concentration of the two. How would I be able to compare red fluorescence intensity in each particular pixel of the first picture to green fluorescence density in each pixel of the second picture?
Please let me know if my description is not well explained or if there is any way I can better explain my situation.
Thank you for your time,