I am attempting to compare images of slides that are stained with different colors (green, red and blue)
My goal is to compare the green stain in one image to another without the other colors interfering and it to be the entire image and not pixel by pixel.
The images are not the same so they can not be overlapped.
I want to be able to show in a quantitative way that one image has more green or that the green is more intense than the other image.
I have tried turning the image to greyscale but then I can’t compare the green stain.
I have tried running the align module and image math but haven’t gotten the results I’m looking for.
I am new to cellprofiler and would appreciate any help.