Hi, I’m having some trouble figuring out the best way to combine a multichannel image with a single channel image. For each field of view, I took a single 4 channel image followed by a time series of a single channel. The channel I’m most interested in is channel 0 in both files, and the other 3 channels in the first file are used for localization and identification. I would like to make a single file from each pair of multi-channel image & single-channel time series. The first frame of channel 0 should be from the multi-channel file, and all subsequent frames in channel 0 should be from the single-channel time series. In all frames, I need channels 1, 2, and 3 to be duplicates of channels 1-3 from the multi-channel image.
Thank you in advance!