I would like to define a gate with a curved and elongated shape on a scatter Plot (x, y) = (MedianRadius x AreaShape_Area) on images of nuclei stained with DAPI.
To clarify, this is somethiong routinely performed by people analysing flow cytometry data (but I need the microscope resolution for some of my markers so I cannot do this analysis by Facs).
Even if gates of complicated shapes cannot be done in CPA, I possibly could combined small square gates, or negatively considere some gates. I read on 2011 and 2012 post of this forum that this was envisonned at the time.
Any suggestion would help…