I’m doing live imaging of drosophila brains, speciffically stem cells, in a Andor spinning disk microscope. My videos have between 5h and 15h and I’m new working with fiji so I have some doubts!
1st- How can I combine all the different “movie” files (they are snaps of the various z’s that I selected), per order, in one movie that shows all the live imaging period?
2nd- The time in the movies does not correspond to the real time of the live imaging (the spinning took snaps at every 3 minutes), being much more accelerated. How can I know?
The thing is that I’m trying to characterize cell division so I need the precise time to be able to follow the rate of division and see if my desire cells are sincronized…
Thanks in advance, I’m very dumb at this!