Color Threshold Histology



Hi! I am new in ImageJ, so please be kind! I am trying to quantify black stained fibers on histology images like the one attached. Is that possible using the threshhold tool?



Welcome to ImageJ and the Forum!

So… perhaps you can clarify a bit more…

  1. What are the ‘black stained fibers’ you are trying to Segment?
  2. When you say “quantify” - what exactly do you want to measure? Area? Curvature? etc.

You can try the Color Threshold tool - that potentially could work… You can also try the Fiji plugin Trainable Weka Segmentation. :slight_smile:

Too - if you are really just getting started with ImageJ/Fiji - here are some helpful links: