I’ve used your sample for colony counting for E.Coli plates. I have tried several plates and the script seems always to over-estimate the number of colonies and it includes also the bubbles on the plate. Are there any parameters that need to be changed. Or maybe the quality of the pictures it’s not good? Could you please provide some tips (I attached a sample plate with around 40 colonies counted manually)?
Thanks a lot,
40colonies.zip (731 KB)