I am very much new to CellProfiler and have been trying to adapt the ExampleYeastPatches pipeline to use for counting my plates. I am using a serial dilution and spotting technique to counting starting CFU/ml of the respective sample. Each row is a separate sample and gets diluted across the plate. The instrument then spots 5ul of a chosen dilution onto a rectangular agar plate.
I need an more automated / high-throughput means to count the resulting colonies, other than counting each by eye, as I am doing currently. Does anyone have input for me? I am going cross-eyed trying to determine which module to use and in what order to result in a countable image.
I have attached an image to use as an example of the type of format from which I need CellProfiler to count colonies.
I appreciate any assistance you can provide.
Picture1.tif (3.0 MB)