Colocalization & quantification

wt glua1 homer 1 composit.tif (1.5 MB) I want to perform an analysis for the attached image for a revision.The reviewers want to know the extent of colocalization and quantitative presentation in this regard to differentiate synaptic and extra synaptic green puncta clusters.I have to use Fiji 2.1.0/1.53 sc installed on MacBook pro.I need help as to what kind of analysis could answer both of the questions? My supervisor doesn’t think a mere colocalization analysis is the answer here. Previously we just counted the green puncta in cell interior between two groups without taking the red channel into account.

I am unable to upload my image here…Can somebody help me in this regard?