I am trying to get quantitative data from IF images of KIT (red) and Rab 7, EEA1 and GM130 after drug treatment. I am trying to determine the quantitative colocalization of KIT receptor in this these compartment versus the quantity the amount of KIT present in the rest of the cells. I have tried to build a pipeline but I am not very gifted, the software loads the images then thresholds, but I don’t really know how to set the parameters correctly in the software…
I would greatly appreciate any help
Thanks a million,
PS: I have attached one of my images.