Hi, I’m new to CellProfiler and overwhelmed with the vast array of measurements available…so i’m trying to tackle one at a time!
First, I’ve labeled tissue sections with multiple fluorescent markers, one of which being DAPI to identify total cell nuclei.
- I want to create a pipeline that measures the colocalization of targets (ER and PR) as well as determine the labeling index of these targets to the total cell population (DAPI).
Secondly, I’m interested in comparing specific cell compartments (Epithelium and Stroma) with which I’ve labeled with Cytokeratin 8, an antibody specific for epithelium.
- How can I do the above mentioned tasks (1.) within each cell compartment?
Thank you for your help, I look forward to any suggestions!!!