I am having a hard time figuring out how to analyse colocalisation of two extracellular proteins.
They both look like those nets and I would like to asses the number of nets, where they are colocalized. However, when I want to do object based colocalization in JACoP, I have problem when setting particle size. Single nets are not discrete and they touch… JACoP cannot recognize them as separate objects. Using ROIs would help, but JACoP don’t use ROIs.