Colocalization analysis - JACoP and segmentation of the image

Hi,
I am having a hard time figuring out how to analyse colocalisation of two extracellular proteins.


They both look like those nets and I would like to asses the number of nets, where they are colocalized. However, when I want to do object based colocalization in JACoP, I have problem when setting particle size. Single nets are not discrete and they touch… JACoP cannot recognize them as separate objects. Using ROIs would help, but JACoP don’t use ROIs.

Thank you.