I have imaged the zymosan particles (Green), to calculate the Pearson coefficient, colocalisation with nanoparticles (Red)
The process is:
1- Subtract the background from both channels (Red and green)
2-Analyse —> colocalisation ------> Coloc 2
3- I put Red channel ana green channel
4- Threshold regression ----Costes
5- then OK
However, the merge images show that the particles phagocytosed with macrophage with nanoparticles, the result of colocalisation shows weak colocalisation?
Is the image processing in the right way?