I have labelled astrocytes in the red channel and my protein of interest in the green channel. I would like to see whether the expression levels of the protein (green channel) expressed on astrocytes are different between groups.
I would therefore like to do a colocalisation analysis but I am not sure of the best way to do this? I am using imagej. This protein is expressed on other cells too, but my aim is to quantify the amount of the protein which is colocalised only on the astrocytes (so not to get a value of how much overlap there is).