Coloc2, multiple ROIs

imagej

#1

Hi guys,

I’m using coloc2 to measure colocalisation on images with multiple cells on. I’ve tried using multiple ROIs on the images I’m analysing, but seem to only get one output (and not one for each ROI/cell). Is there a way of making coloc2 give you the values for each ROI on an image separately, without having to use one just one ROI at a time (which I’m currently doing, and it’s taking forever)?

Many thanks!
Ane


#2

@astranger

The only way to do one ROI at a time would be to write a script. To get started with scripting in ImageJ:

Also - there were some recent Coloc scripting questions here on the forum that you can take a look at to also get you started:


eta :slight_smile:


#3

Excellent, thank you very much!

Ane :slight_smile: