I’m trying to make a co-localization pipeline which in my mind should be very easy to make but I just can’t make it work. I really hope for some good advice.
I have cells stained with:
CD8 antibody (RED)
KV13 antibody (GREEN)
DAPI nuclei (blue)
I have the following goals that I would like to achieve:
- Calculate the percentage of All cells (blue) that are CD8 positive.
- Calculate the percentage of CD8 cells (red) that are KV1.3 positive (green) - in the picture it is the yellow ones.
- Calculate the intensity of the fluorescence - to get a quantitative measure of protein level in the cell (this goes for the KV1.3 positive cells).
In my pipeline I’ve managed to get the CD8 identified as primary objects and also KV1.3 (green) cells as primary objects. I have tried the relate command to get some “ratio” of the red cells that also where Green, but this doesn’t give me a number or ratio?
I 'm looking forward solving these issues
DefaultOUT.mat (57.6 KB)