I started to create a pipeline to colocalize a green and a red signal expressed in cells, largely sticking to the examples and tutorial given here. In principle the pipeline works and I think I create all the info I would like to have, but I do not seem to get it out!
The problem is that I would like to get the number of colocalized objects for each cell, but so far I get all kind of info for each image (each with several cells) or info for each object, but not the info on objects colocalized per cell.
I attach my presumably too complicated pipeline (as I am not sure what I might omit), if someone has an idea I would be grateful…
Coloc-170613.cp (19.6 KB)