I have stained for BDNF and p-MAPK (phosphorylated-MAPK). BDNF is a ligand and when it binds to TrkB(receptor), there are multiple down stream process.
p-MAPK is one of the downstream protein of BDNF-TrkB interaction.
Now, I want to do a correlation analysis between BDNF and p-MAPK and I do not want to proceed with co-localization because these 2 proteins don’t really overlap as p-MAPK is a downstream protein. Are you aware of any correlation software that would help us in this analysis. Also, is there any other useful analysis that gives us the information about their interaction.