Wondered if you could help!
I’m trying to calculate the proportion of GFP +ve cells which are also positive for EdU in an image. Ive also used DAPI as a nuclear stain.
Ive set up a pipeline to identify primary objects (EdU and DAPI) and then secondary objects (for the GFP), using DAPI as the input object. The problem is that not all of my DAPI positive cells are also positive for GFP but cell profiler seems to be calculating them as such. I’m not sure what I need to change (is it some thing in the Identidy Secondary Objects part?).
Any help would be much appreciated