I take avi file for cilia beating frequency data analysis.
When I convert the cine (original format) to avi, I set the fps to 15fps and save in avi.
Then, I drop the avi files in ImageJ/Fiji with macro for beating frequency in the unit of beats/sec.
For this steps, I get make graph in excel using the results from ImageJ/Fiji then count the peaks. For example, peak1:200, peak2:300, peak3:450
calculation I do is:
- 450-300=150, 300-200=100
- 150+100/2= 125 frames/beat
- Convert it to beats/sec: 200frames/secX1beat/125 frames=1.6 beats/sec
My concerning part is that step 3) converting, multiplying 200 frames/sec to get beats/sec unit.
Does it have to be 15fps that this is the f/s I chose when I converted the cine file to avi at the beginning?
Help will be appreciated a lot.