I’m new here and I’ve found the program to be excellent.
I have a pipeline in cell profiler 2.0 that is working very well for counting foci per nuclei, however I can only seem to get the output of mean child object per parent- for each image.
The mean per image is not really any help for quantification (statistical analysis)- I really need the counts for each parent object (as these numbers will vary between images) before I can perform any analysis.
Is there a way to get values (number) of child objects for EACH parent object in each image- not the mean per image (in the exported data)?
Is that possible? Any help is much appreciated.
All the best,