Dear CellProfiler Team,
I am currently trying to establish a pipeline that recognises and characterises megakaryocytes (progenitors of blood platelets) according to their morphology. These cells are cool but quite annoying, because they can be nicely round, or spread like a fried-egg, or irregularily formed with pseudopodia like structures, or fragmentated and finally they can also extend long thin branching processes (proplatelets). Of course they also flow into each other to be even more annoying^^
I would like to differentiate them into:
undifferentiated, round cells
spread, relatively round cells with a surface:nucleus ratio of more than 10:1
proplatelet forming cells (long, thin and branching processes)
cells that are between spread and proplatelet forming cells
As fully differentiated cells lose their nucleus I cannot use nuclei aone as primary objects, therefore I pre-processed my images with ImageJ and merged the dapi and tubulin images. Tubulin nicely outlines the CD41 stained complex cell body. Furthermore I separated critical cell cell junctions manually with a black line.
Now I am struggling with FilterObjects though, and I have no clue how to solve this problem^^ I would like to exclude objects that have a surface:nucleus ratio greater or equal than 10:1 AND that are in my upstream classified AreaShape_FormFactor from Bin 1 to Bin 5. The first parameter works well, I don’t get how to set up the second condition though…
And do you have any suggestions how I could define fragmentated cells?
Thank you very much in advance for your help and I hope that I explained my problem well enough^^
cell profiler project.cpproj (103 KB)