I have been using CP 2.1.1 and CPA, with Windows, and am very happy !!!
Lately I have been trying to classify 8-bit images, that a collaborator gave me, and have 2 channels/ colours. CP works fine, but when I subsequently ‘Fetch’ images in CPA, the thumbnails are black and get a WARNING.
WARNING: CPA found 3 channels in the “Phalloidin” image at xxxx.tif, but it will only load the first 1 as specified by properties field channels_per_image.
I don’t understand why it says that I have ‘3 channels’. I tried to save the images differently, but still get the same WARNING and black thumbnails.
As a solution I decided to go with it and edit the properties file, as follows, but not the CPA will not launch at all. Please note that I do not have ‘dna’ channel/colour, only ‘DAPI’, and ‘Phalloidin’. I was just trying to see if it would work – and it didn’t…
Please help !
image_path_cols = Image_PathName_DAPI,Image_PathName_Phalloidin
image_file_cols = Image_FileName_DAPI,Image_FileName_Phalloidin
image_thumbnail_cols = Image_Thumbnail_DAPI,Image_Thumbnail_Phalloidin
image_names = DAPI,Phalloidin
image_channel_colors = red, green
image_path_cols = Image_PathName_DAPI,Image_PathName_Phalloidin, Image_PathName_dna
image_file_cols = Image_FileName_DAPI,Image_FileName_Phalloidin, Image_FileName_dna
image_thumbnail_cols = Image_Thumbnail_DAPI,Image_Thumbnail_Phalloidin, Image_Thumbnail_dna
image_names = DAPI, Phalloidin, dna
image_channel_colors = green, blue, gray
channels_per_image = 3
image_channel_blend_modes = add, add, add
Thank you, in advance !