I am in the midst of analyzing fluorescent micrographs, and I want to measure fluorescence intensity of numerous regions in the same micrograph.
I am currently using the Point tool + Measure to record a single pixel’s information, but I want to expand it to a 2-dimensional circular area of constant size. I know that I can use the ‘Oval Selection’ tool repeatedly, but since it is a scalable tool, I am finding it difficult to draw perfect circles of identical radii in an efficient way. I want to measure many dozens of points per micrograph, and have about 100 micrographs to analyze.
Ideally, I want to be able click on a particular spot and for it to automatically draw a circular area of a defined (constant) size repeatedly. I’m completely new to macros and the like, and haven’t figured out a way of doing this.
If anyone has any ideas please let me know!