I’ve been trying to segment a component within my image but I think the contrast is not sufficient for the threshold method to work. An example of the image is here
you might see in the image some circular ish regions (which is not the gap in the middle, that gap is an artery with a vein besides) surrounded by bundles.
The circular regions are pores within the tissue surrounded by bundles of collagen. a schematic of the image is here
in this image you see the circular pores and the surrounding bundles.
I am trying to segment the circular regions from the rest of the image (from everything else).
From the first image I upload it’s very tricky, i tried to apply different filters but nothing, the threshold method cannot pick up the circular pores as they grey level is very similar to that of the surrounding everything else.
does anybody know a way of doing this?
I would like to obtain something like this
where all the circular regions are black and separated from everything else.
I spent lots of time on this but nothing good came up.