I would like to measure the intensity of moving cells (that I previously tracked with trackmate) over time (I work on a timelapse file of around 150 frames that I split in 4 channels).
I tried to do that using the tools of trackmate but when I “copy the overlays” from one channel to another and that I look at the intensity table, values are the same for my 4 channels while they should be different. My four channels correspond to four stainings. They should appear at different moments in cells, and cell’s intensity should vary according to the channel. That’s why I would like to know if there is a way to measure channel-specific intensity…
Many thanks in advance for your help!!